Investigation of bone microarchitecture and histomorphometry
HiMolA offers its service in conventional bone histomorphometry including bone preparation (calcified and decalcified) and embedding, sectioning, and staining. This platform routinely performed several stainings for the identification of bone marrow cells (toluidine blue-borax), osteoclasts (tartrate-resistant acid phosphatase), osteoblasts (alkaline phosphatase), osteoid tissue (Goldner trichrome) or metal accumulation (Perl’s, solochrome azurine). Static morphometric parameters, indicative of the structure and activity at the time-point of the collection of the bone specimen, can be measured according to the American Society for Bone and Mineral Research Guidelines. Dynamic parameters, indicative of the amount of bone formation between two given time points, can also be determined. In addition, the platform has also expertise in performing molecular detection by immunohistochemistry of bone samples and analyses of such samples.
The platform is also specialized in X-ray imaging of calcified tissue by micro/nanoCT and propose several solutions ranging from in-vivo investigation of bone microarchitecture in small animals to ex-vivo analysis of specimen in three dimensions (3D). These solutions are useful in determining volumetric bone mineral density and to characterize the microarchitecture of bone specimen.
The platform is equipped with :
- Leica Polycut
- Struers Accutom-50
- Struers Labotom-3
- Exakt saw
- Exakt polishing machine
- Struers Tegramin-30
- Fume hood
- Faxitron LX-60 cabinet
- Skyscan 1076 in vivo microCT
- Skyscan 1272 ex vivo microCT
- GE Phoenix nanotom S nanoCT
